CaSiPaC - Calcium Signaling during Parabolic Flight in Chondrocytes

Cytosolic free calcium and membrane potential recordings under altered gravity during a parabolic flight.

Brief information

School:

Engineering and Architecture

Status:

Completed

Period:

01.05.2020 - 31.12.2021

Overview

Chondrocytes are the sole resident cells in articular cartilage, which line the articulating bones in joints allowing effortless movements. Chondrocytes are responsible for cartilaginous matrix synthesis, maintenance and degradation. Since these cells are frequently exposed to mechanical loading patterns, it is generally believed that chondrocytes require mechanical stimuli for adequate cartilage homeostasis. In this context, mechanosensitive ion channels are thought to be among the first instances in cellular mechanosensing. Prior studies showed that applied mechanical stimuli increase cytosolic free calcium and hyperpolarization of the cells’ membrane potential. We examined during a parabolic flight whether cytosolic free calcium and membrane potential are gravity-dependent. Our experiments showed that both cytosolic free calcium and membrane potential remain unchanged in response to short periods of increased or reduced gravity.

Facts

Type of project

Forschung

Internal organisations involved
  • CC Bioscience and Medical Engineering
External project funder
Funding
  • Ausland

Persons involved: internal

Project manager
Member of project team

Publications

    • Hammer, Andreas; Cerretti, Geraldine; Ricciardi, Dario A.; Schiffmann, David; Maranda, Simon; Kummer, Raphael; Zumbühl, Christoph; Rattenbacher-Kiser, Karin; von Arx, Silvan; Ammann, Sebastian; Strobl, Frederic; Berkane, Rayene; Stolz, Alexandra; Stelzer, Ernst H.K.; Egli, Marcel; Schleiff, Enrico; Wuest, Simon L. & Böhmer, Maik (2022). Retrograde Analysis of Calcium Signaling by CaMPARI2 Shows Cytosolic Calcium in Chondrocytes Is Unaffected by Parabolic Flights. Biomedicines, 1.

    • Bradley, Timothy; Cerretti, Geraldine; Egli, Marcel; Follonier, Cindy; Ille, Fabian; Rattenbacher-Kiser, Karin; Wadsworth, Jennifer Louise & Wüest, Simon (2022). Cytosolic calcium and membrane potential in articular chondrocytes during parabolic flight. Acta Astronautica, 2022(193), 287-302.